Detection of Ehrlichia chaffeensis DNA in Amblyomma americanum ticks in Connecticut and Rhode Island.
نویسندگان
چکیده
Ehrlichia chaffeensis, the causative agent of human monocytic ehrlichiosis, is transmitted by Amblyomma americanum ticks, which are most abundant in the southern United States. Because serologic evidence suggests that residents of Connecticut are exposed to E. chaffeensis, A. americanum ticks were collected in Connecticut and Rhode Island for PCR analysis to detect E. chaffeensis DNA. Eight of 106 (7.6%) A. americanum ticks from Connecticut and 6 of 52 (11.5%) from Rhode Island contained E. chaffeensis DNA. Thus, E. chaffeensis is present in ticks in southern New England and transmission of E. chaffeensis may occur there.
منابع مشابه
Prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii in ticks from Tennessee.
Human ehrlichiosis is the second most common tick-borne disease reported in Tennessee after Rocky Mountain spotted fever. Two closely related ehrlichiae, Ehrlichia chaffeensis and Ehrlichia ewingii, are both causative agents of human disease and are transmitted by Amblyomma americanum, the lone star tick. Prevalence rates and distribution patterns of these pathogens among ticks in Tennessee are...
متن کاملInfection rates of Amblyomma americanum (Acari: Ixodidae) by Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) and prevalence of E. chaffeensis-reactive antibodies in white-tailed deer in southern Indiana, 1997.
To monitor the percentage and stability of Ehrlichia chaffeensis-infected ticks in southern Indiana over time, pools of Amblyomma americanum (L.) ticks were screened for infection in southern Indiana for a 2nd time. Nested polymerase chain reaction (with 6% DMSO included only in the 2nd reaction) was performed on 920 ticks in pools of 5 individuals from 9 sites (5 sites previously examined and ...
متن کاملSensitive detection of Ehrlichia chaffeensis in cell culture, blood, and tick specimens by reverse transcription-PCR.
Ehrlichia chaffeensis is an obligatory intracellular bacterium of monocytes and macrophages and the etiologic agent of human monocytic ehrlichiosis, an emerging zoonosis. The Lone Star tick (Amblyomma americanum) has been implicated as the primary vector of E. chaffeensis. The present study examined the sensitivity of the nested reverse transcription (RT)-PCR based on the 16S rRNA gene relative...
متن کاملSite-specific geographic association between Amblyomma americanum (Acari: Ixodidae) infestations and Ehrlichia chaffeensis-reactive (Rickettsiales: Ehrlichieae) antibodies in white-tailed deer.
Serum samples from white-tailed deer, Odocoileus virginianus Zimmermann, collected from 1982 through 1992 from the southeastern United States were tested for antibodies reactive to Ehrlichia chaffeensis Anderson, Dawson, Jones, & Wilson, the causative agent of human ehrlichiosis. Results were compared between areas based on known infestations of the lone star tick, Amblyomma americanum L., a su...
متن کاملDistribution of Ehrlichia chaffeensis (Rickettsiales: Rickettsiaeceae) in Amblyomma americanum in southern Indiana and prevalence of E. chaffeensis--reactive antibodies in white-tailed deer in Indiana and Ohio in 1998.
To continue monitoring the prevalence and distribution of Ehrlichia chaffeensis (Rickettsiales: Rickettsiaeceae) in southern Indiana, a total of 498 Amblyomma americanum (L.) ticks (262 adults and 292 nymphs) was collected from five southern Indiana counties during May and June 1998. Ticks were pooled and examined for the presence of E. chaffeensis using nested polymerase chain reaction and pri...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 38 12 شماره
صفحات -
تاریخ انتشار 2000